Our collaborative efforts at IRF and NIH was resulted in a Nature Communications paper, for which Wing-Pui Kong and Barney S. Graham were the corresponding authors, and I was closely worked with Dr. Kayvon Modjarrad and Dr. Jeff Solomon.
Evaluation of candidate vaccine approaches for MERS-CoV
In this study, we showed that immunogens based on full-length S DNA and S1 subunit protein elicit robust serum-neutralizing activity against several MERS (middle east respiratory syndrome coronavirus) strains in mica and non-human primates. Serological analysis and isolation of murine monoclonal antibodies revealed that immunization elicits NAbs to RBD and, non-RBD portions of S1 and S2 subunit. Multiple neutralization mechanisms were demonstrated by solving the atomic structure of a NAb-RBD complex, through sequencing of neutralization escape viruses and by constructing MERS-CoV S variants for serological assays. Immunization of rhesus macaques confers protection against MERS-CoV-induced radiographic pneumonia, as assessed using computerized tomography, supporting this strategy as a promising approach for MERS-CoV vaccine development.
Here is the PDF of the article